Constrained spheroids for prolonged hepatocyte culture

Wen Hao Tong^{a, b, c}, Yu Fang^{a, b, c}, Jie Yan^{a, c, g}, Xin Hong^c, Nisha Hari Singh^a,
Shu Rui Wang^d, Bramasta Nugraha^{a, b, e, f}, Lei Xi^{a c}, Eliza Li Shan Fong^c,
Ciprian Iliescu^a, Hanry Yu^{a, b, c, g, h, i},
^a Institute of Biotechnology and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore
^b NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), #05-01, 28 Medical Drive, Singapore 117456, Singapore
^c Department of Physiology, Yong Loo Lin School of Medicine, MD9-03-03, 2 Medical Drive, Singapore 117597, Singapore
^d Bruker Singapore Pte Ltd., 11 Biopolis Way #10-10, The Helios, Singapore 138667, Singapore
^e ETH Zürich, Department of Biosystem Science and Engineering (D-BSSE), Mattenstrasse 26, CH-4058 Basel, Switzerland
^f Roche Pharmaceutical Research and Early Development, Department of Discovery Technology, Roche Innovation Center Basel, Grenzacherstrasse 124,
CH-4070 Basel, Switzerland
^g Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Tower, Singapore 138602, Singapore
^h Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411, Singapore
ⁱ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Biomaterials (2016) 80 106e120

Samco Basic Plasma Kit was used for surface oxidation of Parylene C membrane in O2 plasma in sample preparation.